Abstract
The effects of COD/NO3- ratio, nitrate concentration and salinity was tested on the performance of the CRBR in denitrification with catechol as carbon source. The maximum nitrate reduction attained at COD/NO3- ratio of 1. The CRBR operated at optimum COD/NO3- ratio could completely denitrify the nitrate at inlet concentration up to 1250mg/L without nitrite accumulation. The maximum denitrification rate in the CRBR was 3.56kgNO3-/m3d with a nitrate reduction efficiency of 99% when the bioreactor was operated at inlet nitrate loading rate of 3.6kgNO3-/m3d. The denitrification performance of the CRBR was not affected significantly by NaCl concentrations up to 20g/L. 16S rRNA fragment and phylogenetic analysis identified Pseudomonas resinovorans, Stenotrophomonas maltophilia and Bacillus cereus as the most abundant denitrifiers in biomass. Accordingly, the CRBR is a high-rate bioreactor and appropriate technology for treatment of nitrate-laden industrial wastewaters containing phenolic compounds and salinity.
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