High quantum yield carbon dots and nitrogen-doped carbon dots as fluorescent probes for spectroscopic dopamine detection in human serum.

Abstract

Recent advances in fluorescent carbon dots have shown great potential for the sensing of biological molecules. In this study, one-step hydrothermally synthesised carbon dots (CD) and nitrogen doped carbon dots (NCD) with high quantum yields of 54.29% and 89.82%, respectively, were investigated and demonstrated to be a reliable, cost-effective, and naked-eye fluorescent probe for the detection of dopamine, a neurotransmitter, in human serum fluids. The current study is well supported by a comprehensive synthesis approach and has been described utilizing a variety of microscopic and spectroscopic techniques. The discovered approach is time and pH dependent, and it provides a robust platform for specifically detecting aberrant dopamine levels using a fluorescence quenching mechanism. Dopamine detection limits for CD were calculated to be 5.54 μM for CD and 5.12 μM for NCD, respectively. The fluorescence quenching shows a linear continuous trend with a range within 3.3-500 μM and 3.3-400 μM of dopamine concentration for CD and NCD respectively. To further verify the sensitivity of CD and NCD as fluorescent probes, interference studies in the presence of different biological components were also studied and validated. This work shows that carbon-based nanomaterials and their doped nanostructures, due to their high fluorescence, have significant potential as fluorescent probes in neurological disease diagnosis as they display high selectivity, sensitivity and fast responses in the real time spectroscopic detection of dopamine in human fluid samples.