Abstract

Plasmodium coatneyi is a protozoan parasite species that causes simian malaria and is an excellent model for studying disease caused by the human malaria parasite, P. falciparum. Here we report the complete (nontelomeric) genome sequence of P. coatneyi Hackeri generated by the application of only Pacific Biosciences RS II (PacBio RS II) single-molecule real-time (SMRT) high-resolution sequence technology and assembly using the Hierarchical Genome Assembly Process (HGAP). This is the first Plasmodium genome sequence reported to use only PacBio technology. This approach has proven to be superior to short-read only approaches for this species.

Highlights

  • A Plasmodium genome sequence was published initially in 2002, for P. falciparum [1]

  • A preliminary draft of the P. coatneyi genome based on short-read (Ͻ500 bp) sequence technology is available in the NCBI database (PRJNA233970)

  • The error correction module was defined as minimum subread length of 100 bp, a minimum read quality of 0.80, and a minimum read length of 6,000 bp

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Summary

Introduction

A Plasmodium genome sequence was published initially in 2002, for P. falciparum [1]. Genome sequences for several other primate Plasmodium species have followed [1,2,3,4,5,6], but none has yet been generated using only PacBio technology [6]. A Plasmodium genome sequence was published initially in 2002, for P. falciparum [1]. Genome sequences for several other primate Plasmodium species have followed [1,2,3,4,5,6], but none has yet been generated using only PacBio technology [6]. Plasmodium coatneyi, which infects Macaca mulatta (rhesus macaques) and serves as a model of P. falciparum [7, 8], was chosen as a test platform for PacBio sequencing.

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