High-quality-draft genome sequence of the heavy metal resistant and exopolysaccharides producing bacterium Mucilaginibacter pedocola TBZ30T

Xia Fan,Gejiao Wang,Jingwei Tang,Jing Huang,Li Nie

doi:10.1186/s40793-018-0337-8

Abstract

Mucilaginibacter pedocola TBZ30T (= CCTCC AB 2015301T = KCTC 42833T) is a Gram- negative, rod-shaped, non-motile and non-spore-forming bacterium isolated from a heavy metal contaminated paddy field. It shows resistance to multiple heavy metals and can adsorb/remove Zn2+ and Cd2+ during cultivation. In addition, strain TBZ30T produces exopolysaccharides (EPS). These features make it a great potential to bioremediate heavy metal contamination and biotechnical application. Here we describe the genome sequence and annotation of strain TBZ30T. The genome size is 7,035,113 bp, contains 3132 protein-coding genes (2736 with predicted functions), 50 tRNA encoding genes and 14 rRNA encoding genes. Putative heavy metal resistant genes and EPS associated genes are found in the genome.

Highlights

The genus Mucilaginibacter was first established by Pankratov et al in 2007 and the type species is Mucilaginibacter paludis [1]
We show that strain TBZ30T is resistant to multiple heavy metals and remove Zn2+ and Cd2+
Genome information Genome project history M. pedocola TBZ30T was sequenced on the basis of its abilities of heavy metals resistance and removal, which has a great potential for bioremediation

Summary

Introduction

The genus Mucilaginibacter was first established by Pankratov et al in 2007 and the type species is Mucilaginibacter paludis [1]. The common characteristics of this genus are Gram-negative, non-spore-forming, nonmotile, rod-shaped and producing exopolysaccharides (EPS) [1, 2]. We show that strain TBZ30T is resistant to multiple heavy metals and remove Zn2+ and Cd2+. Strain TBZ30T showed the highest 16S rRNA gene sequence similarity with Mucilaginibacter gynuensis YC7003T (95.8%), Mucilaginibacter mallensis MP1X4T (95.4%) and Mucilaginibacter litoreus BR-18T (95.4%) [6–8] and grouped together with M. gynuensis YC7003T (95.8%) and M. mallensis MP1X4T (Fig. 1). Strain TBZ30T could adsorb/remove nearly 60% of Zn2+ and 55% of Cd2+ in the R2A liquid medium (added with 0.3 mM ZnSO4 and 0.25 mM CdCl2, respectively) (Fig. 4). Genome information Genome project history M. pedocola TBZ30T was sequenced on the basis of its abilities of heavy metals resistance and removal, which has a great potential for bioremediation. The quality and quantity of the DNA were determined using a spectrophotometer (NanoDrop 2000, Thermo)

Sample collection

Libraries used

Not in COGs

Capsular biosynthesis protein PHP

Findings

Conclusions