Abstract
ABSTRACTThe high-quality draft genome of “Candidatus Methanoperedens sp.” strain BLZ2, a nitrate-reducing archaeon anaerobically oxidizing methane, is presented. The genome was obtained from an enrichment culture and measures 3.74 Mb. It harbors two nitrate reductase gene clusters, an ammonium-forming nitrite reductase, and the complete reverse methanogenesis pathway. Methane that escapes to the atmosphere acts as a potent greenhouse gas. Global methane emissions are mitigated by methanotrophs, which oxidize methane to CO2. “Candidatus Methanoperedens spp.” are unique methanotrophic archaea that can perform nitrate-dependent anaerobic oxidation of methane. A high-quality draft genome sequence of only 85 contigs from this archaeon is presented here.
Highlights
The high-quality draft genome of “Candidatus Methanoperedens sp.” strain BLZ2, a nitrate-reducing archaeon anaerobically oxidizing methane, is presented
Contigs were binned using MetaBAT [2], yielding a draft genome composed of 85 contigs (N50 ϭ 74.304 bp)
All genes necessary for reverse methanogenesis were present in the genome, including mer
Summary
The high-quality draft genome of “Candidatus Methanoperedens sp.” strain BLZ2, a nitrate-reducing archaeon anaerobically oxidizing methane, is presented. We extracted DNA from a bioreactor highly enriched in “Ca. Methanoperedens sp.” strain BLZ2 using the cetyltrimethylammonium bromide (CTAB) method as described previously [1] and the Power soil DNA extraction kit (Mo Bio Laboratories, Uden, The Netherlands). To shear genomic DNA and add adapters in the same step (“tagmentation”), the Illumina Nextera XT library prep kit (Illumina, San Diego, CA, USA) was used.
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