Abstract
BackgroundComparative genomics is a formidable tool to identify functional elements throughout a genome. In the past ten years, studies in the budding yeast Saccharomyces cerevisiae and a set of closely related species have been instrumental in showing the benefit of analyzing patterns of sequence conservation. Increasing the number of closely related genome sequences makes the comparative genomics approach more powerful and accurate.ResultsHere, we report the genome sequence and analysis of Saccharomyces arboricolus, a yeast species recently isolated in China, that is closely related to S. cerevisiae. We obtained high quality de novo sequence and assemblies using a combination of next generation sequencing technologies, established the phylogenetic position of this species and considered its phenotypic profile under multiple environmental conditions in the light of its gene content and phylogeny.ConclusionsWe suggest that the genome of S. arboricolus will be useful in future comparative genomics analysis of the Saccharomyces sensu stricto yeasts.
Highlights
Comparative genomics is a formidable tool to identify functional elements throughout a genome
Genome sequence and assembly We generated a high quality genome assembly for S. arboricolus using a combination of high-throughput sequencing platforms and strategies (Table 1)
Our approach of using multiple high-throughput sequencing strategies resulted in high quality genome sequence that continuously covers the large majority of the S. arboricolus genome
Summary
Comparative genomics is a formidable tool to identify functional elements throughout a genome. In the past ten years, studies in the budding yeast Saccharomyces cerevisiae and a set of closely related species have been instrumental in showing the benefit of analyzing patterns of sequence conservation. Comparative genomics have revealed evolutionary mechanisms that shape genomes and provided a formidable tool for assigning function to DNA sequence [1,2]. Combining experimental data for protein binding sites with sequence conservation allowed the identification of functional DNA sequences [8,9]. The power of these and other comparative genomic approaches [10] rely upon the number of species sequenced, the evolutionary divergence of the selected species and the quality of the assembled genome sequence
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