High production of pyoluteorin and 2,4-diacetylphloroglucinol by Pseudomonas fluorescens S272 grown on ethanol as a sole carbon source

Abstract

Pseudomonas fluorescens S272 newly isolated from a soil sample produced a considerable amount of pyoluteorin and 2,4-diacetylphloroglucinol when grown on ethanol as a single carbon source. The coproduction of approximately 150 μg/ml of pyoluteorin and approximately 500 μg/ml of 2,4-diacyl-phloroglucinol was achieved by flask cultutivation in a medium containing approximately 2% ethanol. A high C N ratio and inorganic phosphate limitation in the medium were also important factors to be considered for optimization of production of the antibiotics.