Abstract
BackgroundNeurocysticercosis (NCC), the central nervous system infection by Taenia solium larvae, is a preventable and treatable cause of epilepsy. In Sub-Saharan Africa, the role of NCC in epilepsy differs geographically and, overall, is poorly defined. We aimed at contributing specific, first data for Rwanda, assessing factors associated with NCC, and evaluating a real-time PCR assay to diagnose NCC in cerebrospinal fluid (CSF).Methodology/Principal findingsAt three healthcare facilities in southern Rwanda, 215 people with epilepsy (PWE) and 51 controls were clinically examined, interviewed, and tested by immunoblot for cysticerci-specific serum antibodies. Additionally, CSF samples from PWE were tested for anticysticercal antibodies by ELISA and for parasite DNA by PCR. Cranial computer tomography (CT) scans were available for 12.1% of PWE with additional symptoms suggestive of NCC. The Del Brutto criteria were applied for NCC diagnosis. Cysticerci-specific serum antibodies were found in 21.8% of PWE and 4% of controls (odds ratio (OR), 6.69; 95% confidence interval (95%CI), 1.6–58.7). Seropositivity was associated with age and lack of safe drinking water. Fifty (23.3%) PWE were considered NCC cases (definitive, based on CT scans, 7.4%; probable, mainly based on positive immunoblots, 15.8%). In CSF samples from NCC cases, anticysticercal antibodies were detected in 10% (definitive cases, 25%) and parasite DNA in 16% (definitive cases, 44%). Immunoblot-positive PWE were older (medians, 30 vs. 22 years), more frequently had late-onset epilepsy (at age >25 years; 43.5% vs. 8.5%; OR, 8.30; 95%CI, 3.5–20.0), and suffered from significantly fewer episodes of seizures in the preceding six months than immunoblot-negative PWE.Conclusions/SignificanceNCC is present and contributes to epilepsy in southern Rwanda. Systematic investigations into porcine and human cysticercosis as well as health education and hygiene measures for T. solium control are needed. PCR might provide an additional, highly specific tool in NCC diagnosis.
Highlights
Epilepsy is a common chronic neurological disorder with a median prevalence of 15 in 1000 in sub-saharan Africa [1,2], and from 7 to 49 in 1000 in Rwanda [3,4]
Neurocysticercosis (NCC) is the infection of the brain with larvae of the pig tapeworm (Taenia solium), which results from the ingestion of, e.g., food or water contaminated with the eggs of this helminth
Our study aimed at investigating the prevalence of and risk factors associated with NCC in southern Rwanda
Summary
Epilepsy is a common chronic neurological disorder with a median prevalence of 15 in 1000 in sub-saharan Africa [1,2], and from 7 to 49 in 1000 in Rwanda [3,4]. NCC is the most severe manifestation of cysticercosis, i.e., the infection with the larval stages of Taenia solium [8], and besides the brain, other organs, e.g., skin and muscle, can be affected by cysticerci. Seizures and epilepsy are the most common manifestation of NCC occurring in approximately 80% of patients [9]. Neurocysticercosis (NCC), the central nervous system infection by Taenia solium larvae, is a preventable and treatable cause of epilepsy. In Sub-Saharan Africa, the role of NCC in epilepsy differs geographically and, overall, is poorly defined. We aimed at contributing specific, first data for Rwanda, assessing factors associated with NCC, and evaluating a real-time PCR assay to diagnose NCC in cerebrospinal fluid (CSF)
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