Abstract
Abstract Supplemental methionine was extracted from a feed sample with O.IM HCI and separated by reverse phase or ion-exchange high pressure liquid chromatography and isocratic elution with KH2PO4 buffer solution as the mobile phase. Methionine was detected at 205 nm. The most reliable results were obtained by using reverse phase chromatography, 0.05M KH2PO4 buffer at pH 2.6 as mobile phase, and tyrosine as internal standard.
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