High Pressure Liquid Chromatographic Determination of Saccharides in Corn Sirups: Collaborative Study

Abstract

Abstract A liquid chromatographic method for quantitative analysis of saccharides in corn sirup was investigated. Fructose, glucose, and related saccharides are separated on an Aminex 50W-X4, calcium form, cation exchange column or, alternatively, on an Aminex Q15-S, calcium form, cation exchange column. Water is the solvent. All carbohydrate components in the sirup elute from the column, permitting normalized computation vs. synthetically prepared standards. The corn sirup samples are diluted to an approximate dry substance and injected directly into the chromatograph. When required, interfering ionic material is removed with the aid of a mixed strong acid-weak base ion exchange resin. The reproducibility and repeatability coefficients of variation for fructose (on samples ranging from 15 to 90% fructose) are 0.9 and 0.3%, respectively. The reproducibility and repeatability coefficients of variation for glucose (on samples ranging from 8 to 50% glucose) are 1.4 and 0.5%, respectively. The method has been adopted as official first action.