Abstract
The development of a specific high-pressure liquid chromatographic (HPLC) assay, having a quantitative detection limit of 0.4 μg/ml, is described for plasma indocyanine green. In a preliminary study, the HPLC method demonstrated that the traditional spectrophotometric procedure inaccurately quantitates the dye's degradation in aqueous medium since degradation products that absorb light at 770 nm are formed. In a further study, the spectrophotometric method yielded an erroneously low clearance of indocyanine green in the rabbit. In addition, the HPLC assay points to the possibility of metabolite formation of the dye in vivo.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.