Abstract
Dasiphora fruticosa (basionym Potentilla fruticosa) is a shrub, known in traditional medicine for centuries. Due to the wide range of pharmacological effects, interest and applications of D. fruticosa extracts are continually increasing; however, reports on optimization of extraction conditions are scarce. Herein, a multi-step high-pressure extraction process with increasing polarity solvents was developed to isolate valuable fractions from D. fruticosa leaves. Supercritical CO2 extraction recovered 2.46 g/100 g of lipophilic fraction, rich in polyunsaturated fatty acids. Further, pressurized liquid extractions (PLE) with acetone, ethanol, and water were applied to obtain antioxidant-rich higher polarity extracts. Under optimized PLE conditions, the cumulative polar fraction yield was 29.98 g/100 g. Ethanol fraction showed the highest yield (15.3 g/100 g), TPC values (148.4 mg GAE/g), ABTS•+, and DPPH• scavenging capacity (161.1 and 151.8 mg TE/g, respectively). PLE was more efficient than conventional solid–liquid extraction in terms of extraction time, extract yields, and in vitro antioxidant capacity. Phytochemical characterization of PLE extracts by UPLC-Q-TOF-MS revealed the presence of hyperoside, ellagic acid, among other health beneficial phenolic substances. Τhis study highlights the potential of high-pressure extraction techniques to isolate antioxidant-rich fractions from D. fruticosa leaves with multipurpose applications, including the prevention and treatment of chronic diseases.
Highlights
Over the last years, there have been extensive studies on the role of reactive oxygen species in several inflammatory processes and oxidative stress, and their implications to the pathogenesis of degenerative aging diseases, such as atherosclerosis, neurodegenerative illnesses, and cancer [1]
In the first part of this study, non-polar constituents were isolated from D. fruticosa leaves employing supercritical carbon dioxide extraction
Miliauskas et al using tert-butyl methyl ether reported that the non-polar fraction yield (0.26%) from D. fruticosa blossoms was significantly lower as compared to ethanol (22.3%)
Summary
There have been extensive studies on the role of reactive oxygen species in several inflammatory processes and oxidative stress, and their implications to the pathogenesis of degenerative aging diseases, such as atherosclerosis, neurodegenerative illnesses, and cancer [1]. Interest in plant-derived phenolic substances as potential agents in the prevention and treatment of oxidative-stress related disorders has gained significant scientific attention [2]. The screening of various plants has dramatically expanded our knowledge on novel antioxidant substances, their modes of action, and roles, either as protective/prophylactic substances or as therapeutic molecules [3]. Under-investigated aromatic, ornamental flowers and medicinal plants, gained attention for their potential use as sources of nutraceuticals with bioactive properties [4]. A previous study of our group on aromatic and medicinal plants grown in Lithuania revealed that Dasiphora fruticosa Potentilla fruticosa) exerts a substantial radical scavenging activity [5,6].
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