High plant species diversity enhances lignin accumulation in a subtropical forest of southwest China

Abstract

Plant species diversity (PSD) benefits soil organic carbon (SOC) accumulation, but mechanisms underlying the stimulative effects of PSD on SOC pools have not been well explored, especially in terms of how PSD impacts plant-derived C accumulation. Here, 45 plots covering a natural gradient of PSD ranging from 0.15 to 3.57 (Shannon's diversity index) were selected in a subtropical forest with calcareous soil to determine the pattern of and controls on the variation of plant-derived C as indexed by lignin phenols along with PSD. The absolute contents of lignin phenols ranged from 1.18 to 6.62 mg g−1 soil with an average of 2.48 ± 1.13 mg g−1 soil across the 45 plots. PSD significantly enhanced soil lignin accumulation via three mechanisms. First, PSD benefited lignin accumulation by stimulating plant detritus inputs. Second, PSD directly and indirectly increased reactive minerals, so that enhanced mineral protection of lignin. Third, decrease in microbial C limitation due to increased soil C availability resulted in lowered peroxidase activity and subsequently lignin degradation, which in turn benefited lignin accumulation. Our study provides mechanisms underlying SOC accumulation in response to increased PSD, which may be integrated into Earth system models in order to better predict SOC dynamics under PSD alteration.