High Performance Thin-Layer Chromatographic Analysis of Sugars in Biomphalaria glabrata (Gastropoda) Infected with Echinostoma caproni (Trematoda)

Abstract

High performance thin-layer chromatographic analysis was done on the hemolymph and digestive gland-gonad complex (DGG) of Biomphalaria glabrata snails experimentally infected with the intramolluscan stages of Echinostoma caproni. The major sugars detected in both the DGG and hemolymph of infected and uninfected snails were glucose and trehalose. Quantitative analysis by scanning densitometry showed a significant reduction in glucose in both the hemolymph and DGG of infected snails at 4, 6, and 8 wk postinfection. A similar analysis on trehalose showed that this sugar was significantly reduced at 6 wk postinfection in the hemolymph and DGG of infected snails and could not be detected from these sites in infected snails by 8 wk postinfection. Findings from this study were compared with information on sugars in B. glabrata infected with larval stages of Schistosoma mansoni.