Abstract
Background: High-performance thin-layer chromatography (HPTLC) is an advantageous, modern analytical technique based on the principles of thin-layer chromatography (TLC), which provides essential benefits, such as improved sample application, better and faster separation, and less mobile phase usage. The aim of this work was to develop a simple and rapid HPTLC method that could be used for the identification of meloxicam and piroxicam. Methods: HPTLC. The analysis was carried out using silica gel 60 F254 glass TLC plates and as the mobile phase: hexane: ethyl acetate: glacial acetic acid, in a ratio of 65:30:5 v/v/v. For the standard solution preparation, ethanol was used. Front: 60 mm. Detection was performed at 366 nm. Results: The Rf value for meloxicam was 0.81 and the Rf value for piroxicam was 0.57. The proposed method can be used in the detection of the analyzed compounds in very low concentrations. It was established that the detection limit of meloxicam was 0.04 μg per band and that of piroxicam was 0.05 μg per band. It was also established that the quantitation limit of meloxicam was 0.12 μg per band and that of piroxicam was 0.15 μg per band. Conclusions: The proposed method is simple, sensitive, stable, cost effective, and eco-friendly. It could be used in research or for routine quality control purposes.
Highlights
High-performance thin-layer chromatography (HPTLC) is an advantageous modern analytical technique based on the principles of thin-layer chromatography (TLC) [1,2,3,4]
Compared to classical TLC, HPTLC provides essential benefits, such as improved sample application, higher separation efficiencies, less mobile phase usage, automatization of the drying of the plates, less time required for analysis, minimized exposure to toxic solvents, and reduced possibilities of environmental pollution [1,2,3,4]
We wanted to choose an eco-friendly solvent that posed no danger to the researchers, because, in HPTLC, the only possible interaction between the researchers and chemicals is when the samples are prepared
Summary
High-performance thin-layer chromatography (HPTLC) is an advantageous modern analytical technique based on the principles of thin-layer chromatography (TLC) [1,2,3,4].Compared to classical TLC, HPTLC provides essential benefits, such as improved sample application (automatic or semi-automatic), higher separation efficiencies, less mobile phase usage, automatization of the drying of the plates, less time required for analysis, minimized exposure to toxic solvents, and reduced possibilities of environmental pollution [1,2,3,4].currently, there is a wide variety of analytical techniques for the identification, separation, and quantification of compounds, HPTLC techniques could provide extra advantages; for example, simultaneous analysis of many samples, simple sample preparation, no prior manipulation for solvents (such as filtration or degassing), lower solvent expenditure and no traces of previous analyses (new stationary phase for each analysis) [3].Compared with other methods used in chromatography, such as gas chromatography (GC) and high-performance liquid chromatography (HPLC), TLC is the simplest and least expensive to perform [4,5]. High-performance thin-layer chromatography (HPTLC) is an advantageous modern analytical technique based on the principles of thin-layer chromatography (TLC) [1,2,3,4]. High-performance thin-layer chromatography (HPTLC) is an advantageous, modern analytical technique based on the principles of thin-layer chromatography (TLC), which provides essential benefits, such as improved sample application, better and faster separation, and less mobile phase usage. The aim of this work was to develop a simple and rapid HPTLC method that could be used for the identification of meloxicam and piroxicam. Conclusions: The proposed method is simple, sensitive, stable, cost effective, and eco-friendly
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
