Abstract
HPTLC is an advanced type of planar chromatography used extensively in the recent years for fingerprinting of medicinal plants, products and for screening lichen substances, quantification of active ingredients and herbal drugs, phytochemical and biomedical analysis and also used to check of presence of adulterants in the formulation. It is more sensitive and possible to run more sample in a short period of time, by using small amount of solvent. It is one of the sophisticated instrumental techniques based on the full capabilities of thin layer chromatography. In this paper, a brief discussion of the type of instrument used in HPTLC, its entire approach, and how this procedure is superior to TLC is presented. This article attempts to concentrate on the use of HPTLC by including examples of medications, medicinal drugs, and formulations that have been analyzed using HPTLC.
Highlights
Chromatography has grown in significance and popularity to become a leading type of analysis in instrumental analytical chemistry
High performance thin layer chromatography (HPTLC) is the improved method of thin layer chromatography (TLC) in more optimized way
It is known as planar chromatography or Flat-bed chromatography
Summary
Chromatography has grown in significance and popularity to become a leading type of analysis in instrumental analytical chemistry. High performance thin layer chromatography (HPTLC) basically depends upon the full capabilities of thin layer chromatography (TLC) As it is useful in analysis of qualitative method and it combines the art with quickness at a moderate cost. Kulkarni et al / Journal of Pharmaceutical and Biological Sciences 2021;9(1): qualitative and quantitative analytical tasks, because of its suitability for high-throughtput screening, sensitivity and reliability in quantification of analytes at nanogram levels. It is one of the most applied methods for the analysis in pharmaceutical industries, clinical chemistry, forensic chemistry, biochemistry, cosmetology, food and drug analysis, environment analysis, and other areas.
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