Abstract
Most common prostaglandin analogs, bimatoprost, latanoprost and travoprost, are licensed for the reduction of elevated intraocular pressure in patients with open angle glaucoma and ocular hypertension, but their non approved use as eyelash enhancers is becoming popular, especially in patients with eyelashes hypotrichosis. A fast and sensitive high performance liquid chromatography tandem mass spectrometry method was developed for the measurement of bimatoprost, latanoprost and travoprost in cosmetic serums freely web-sold to increase eyelash length, thickness and darkness. The analytes and the internal standard (reserpine) were separated by reversed phase chromatography with 5 mM ammonium acetate with 0.02% formic acid (mobile phase A) and 5 mM ammonium acetate in acetonitrile/water (95/5; v/v) with 0.02% formic acid (mobile phase B) by gradient elution and detected with tandem mass spectrometry operated in multiple reaction monitoring mode. Linearity between 1 and 500 μg/g shows good correlation coefficients (r2 = 0.99) for all substances. Analytical recovery of analytes under investigation were always higher than 90% and intra-assay and inter-assay precision and accuracy always better than 11%. This method was successfully applied to analyze cosmetic serums freely sold on the Internet websites.
Highlights
Besides providing a natural protective barrier against foreign bodies for the eyes, eyelashes possess an aesthetic function [1,2]
For prostaglandin analogs (PGAs) extraction from cosmetic serum, 100 mg product added with 5 μL of IS (1 mg/mL) were transferred into screw-capped glass tube and 995 μL of HPLC mobile phase (5 mM ammonium acetate with 0.02% formic acid and 5 mM ammonium acetate in acetonitrile/water (95/5; v/v) with 0.02% formic acid, 50:50 v/v) were added
Chromatography was carried out using an kinetex biphenyl 100A (2.1 mm 100 mm, 2.6 μm) using a linear gradient elution with two solvents 5 mM ammonium acetate with 0.02% formic acid and 5 mM ammonium acetate in acetonitrile/water (95/5; v/v) with 0.02% formic acid Solvent B was maintained at 5% for the first 4.0 min
Summary
Besides providing a natural protective barrier against foreign bodies for the eyes, eyelashes possess an aesthetic function [1,2]. Since the beginning of prostaglandin analogs (PGAs) use as ocular trials to stimulate eyelash growth. Since the beginning of prostaglandin analogs (PGAs) use as hypotensive agentsagents for thefor treatment of glaucoma, eyelashes hypertrichosis has been reported as a ocular hypotensive the treatment of glaucoma, eyelashes hypertrichosis has been reported side effect [9,10]. Theeffectiveness effectiveness bimatoprost eyelash growth been demonstrated by clinician ratings, digital image analysis, and patient satisfaction [12,13,14], but the has been demonstrated by clinician ratings, digital image analysis, and patient satisfaction [12,13,14], ability of PGAs to increase eyelash growth does not appear limited to bimatoprost. PGAs,and latanoprost to treat ocular hypertension, haveeyelash been changes, including increases in length and darkness
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