High-performance liquid chromatography of thiazolidinic compounds obtained by condensation of pyridoxal 5′-phosphate or pyridoxal with aminothiols ( l- or d-cysteine, cysteamine, l-cysteine ethyl ester)

Abstract

We investigated six thiazolidine 4-carboxylic acids of biological interest, obtained by condensation of pyridoxal 5′-phosphate or pyridoxal with l- or d-cysteine, cysteamine or l-cysteine ethyl ester. A reversed-phase high-performance liquid chromatographic method, using a C 18 column for their separation, was developed by sequential optimization of the pH and the gradient of the mobile phase. Resolution of the compounds was obtained with an analysis time of less than 20 min.