Abstract
AbstractA sensitive, reversed phase, high performance liquid chromatographic procedure for the analysis of non‐protein amino acids has been developed. The method is based on precolumn derivatization with 9‐fluoroenylmethyl chloroformate of the non‐protein amino acids characteristic of seeds of Acacia species, namely N‐acetyldjenkolic acid, diaminopropionic acid and diaminobutyric acid and their acetyl, ureido and oxalyl N‐substituted derivatives. The separation of derivatives was achieved by means of a gradient elution programme utilizing two buffers of different pH values and acetonitrile. Following separation, the derivatized amino acids were detected spectrofluorometrically. The levels of non‐protein amino acids in seeds of Acacia species, which were representative of three taxonomically distinct groups, were measured by analysis of ethanol extracts.
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