Abstract
The method used previously for the fluorodensitometric determination of compounds with an alcoholic hydroxyl group was examined for its applicability in high-performance liquid chromatography. The conversion of alcoholic substances into urethanes was performed with naphthyl isocyanate. Chloroform—benzene—ethanol and n-heptane—diethyl ether, were used as eluents for the separation of urethanes of various polarity with silica gel columns. Reversed-phase material is also suitable. The detection limits ascertained by means of fluorescence detection are in the picomole range.
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