Abstract
During fermentative production of new carbapenem compounds of the OA-6129 type, many interfering by-products, exhibiting physicochemical properties very similar to the main components, are produced. The compounds of interest, especially OA-6129 A, B1, B2 and C, are difficult to quantify because of the complex biological matrix and the broad spectrum of by-products. High-performance liquid chromatography (h.p.l.c.) is used to quantify OA-6129 B2. In order to assign the chromatographic peaks clearly to the wanted substances, h.p.l.c., t.l.c, and a selective chemical degradation method proved to be necessary. This procedure was considerably simplified by combined use of a column-switching technique and a diode-array detector. The polar by-products and much of the interfering matrix were eliminated by column switching. Positive identification of OA-6129 B2 was feasible by monitoring chromatograms simultaneously at different wavelengths and by use of the diode-array detector. Additional information on uniformity and purity of the peaks was obtained by u.v.-absorbance ratio recording.
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