Abstract
Tramadol is one of widest abusedstreet drug inEgypt. Recently, drug abusers were taking tramadol beside sildenafil for sexual purposes.So it was important to develop a newHPLC method for the simaultineous determination of tramadol andSildenafil in the blood of rats exposed to a combination of specific dose of tramadol and sildenafil. Sample preparation involved liquid–liquid extraction with methyl tertiary butyl ether (MTBE) and backextraction with hydrochloric acid.Tramadol,sildenafiland the internalstandardnalbuphine were separated by reversed phaseHPLCusing60%acetonitrileand 40% 20mMsodium phosphate bufferpH 7.5. Detection was by using UV at wavelengths 230 nm. The method was linear fortramadol (0.8–100ug/ml) and Sildenafil (0.3–100 ug/ml) with mean recoveries of 97.2% and 100.8%, respectively. Intra- and inter-day precisionswere 9.34% and 9.12% for tramadol and 11.82%, and10.43% for sildenafilat the respective limits of quantitation (0.8 and 0.3ug/ml). Accuracy fortramadol and Sildenafil ranged from 96.6% to 97.8%and 99.2% to 101.3% respectively. The method was applied to apharmacokinetic study of tramadol and sildenafil in rats.
Highlights
Tramadol hydrochloride, (±)-Trans-2-[(dimethylamino)methyl]-1-(3-methoxyphenyl) cyclohexanol (Fig. 1a), is acentrallyacting analgesic used in the treatment of mild tomoderate pain [1]
Several HPLC methods have been developed for determination of sildenafil in various samples, such as dietary supplements [5,6,7], pharmaceutical preparation [8], and mouse skin [9], human plasma and urine [10,11,12]
It was found that there were no published methodologies for simultaneous determination of Tramadol, Sildenafil and Intrnal standard from rat blood samples using HPLC–UV,so it was necessary to validate each step of the suggested analytical method.The calibration curves were constructed by plotting the ratioof the peak areas versus concentrations in the working range.Good linearity was achieved for Tramadol and sildenafil in therange studied
Summary
Tramadol hydrochloride, (±)-Trans-2-[(dimethylamino)methyl]-1-(3-methoxyphenyl) cyclohexanol (Fig. 1a), is acentrallyacting analgesic used in the treatment of mild tomoderate pain [1]. Several HPLC methods have been developed for determination of sildenafil in various samples, such as dietary supplements [5,6,7], pharmaceutical preparation [8], and mouse skin [9], human plasma and urine [10,11,12]. For a review see “Sildenafil determination in various matrices” [13] Analyses were based on liquid-liquid extraction at basic pH [6, 7]. For this purpose we used this simple, rapid and accurate HPLC method
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