High Performance Liquid Chromatography (HPLC) Fingerprinting, Mineral Composition and In Vitro Antioxidant Activity of Methanol Leaf Extract of Synsepalum dulcificum (Sapotaceae)

T O Obafemi ,Afolabi C Akinmoladun ,Mary Tolulope Olaleye ,J A Falode ,Amos Onasanya ,Kayode Komolafe ,Aline Augusti Boligon ,Margareth Linde Athayde

doi:10.7324/japs.2017.71117

Abstract

This study was carried out to identify the phytochemicals present and in vitro antioxidant activity of methanol leaf extract of Synsepalum dulcificum (MSD). Standard protocols were used to evaluate the total phenols, total flavonoids and total antioxidants content of the extract. Nitric oxide (NO), hydroxyl radical (OH), ABTS�+ and 2,2-diphenyl -1-picrylhydrazyl (DPPH) radicals scavenging activity, inhibition of lipid peroxidation and the ability of MSD to chelate ferrous ion as well its reductive potential were also evaluated. HPLC was used to confirm the presence of polyphenols and carotenoids. Results showed the presence of flavonoids, saponins, terpenoids and cardiac glycosides in the extract. Among others, appreciable levels of potassium, calcium, sodium and magnesium were detected in the extract. The extract showed a high DPPH, ABTS�+, hydroxyl and nitric oxide radicals scavenging activity. The IC50 of the extract for DPPH, NO, OH-, and ABTS�+ radicals scavenging assays were 139.45 μg/ml, 119.17 μg/ml, 147.65 μg/ml, and 135.83 μg/ml respectively. The total phenol and total flavonoids of MSD were estimated to be 3.244 mg/gTAE and 13.02 mg/gQE respectively. It could be inferred that MSD showed appreciable in vitro antioxidant activity and could be useful in preventing and ameliorating diseases in which free radicals are implicated.

Highlights

In recent times medicinal plants have occupied a unique position among the treatment modalities of a wide variety of diseases
It is believed that the phytochemical composition and the in-vitro antioxidant activity of a plant might give a clue to its therapeutic potential
Egg yolk homogenate served as lipid rich medium, and FeS04 acts as initiator of lipid peroxidation .Briefly, 50μl of plant extract was mixed with 0.25ml 10% egg yolk .This was followed by the addition of 10μl FeSO4(0.07M,aqueous).The mixture was incubated at room temperature for 30min.This was followed by the addition of 0.75ml of glacial acetic acid(5%,v/v aqueous) and 0.75ml of thiobarbituric acid 0.8% in Sodium dodecylsulphate (SDS) 1.1%

Summary

INTRODUCTION

In recent times medicinal plants have occupied a unique position among the treatment modalities of a wide variety of diseases. This might not be unconnected with the presence of biologically active molecules in such plants especially molecules that have been proven to have therapeutic potentials in several. Plant-based antioxidants are preferred to the synthetic ones because of safety concerns (Grice, 1986; Wichi, 1988) These factors have inspired the widespread screening of plants for possible medicinal and antioxidant properties, the isolation and characterization of diverse phytochemicals and the development and utilization of antioxidants of natural origin (Jayaprakasha et al, 2001; Gulcin et al, 2002). To the best of our knowledge the antioxidant activity of the methanol extract of Synsepalum dulcificum leaves has not been evaluated, this study

MATERIALS AND METHODS

DISCUSSION

Result

80 Trolox

Findings

CONCLUSION