Abstract
Reverse-phase high performance liquid chromatography with photodiode array detection (PAD) has beendeveloped for simultaneous separation, identification and quantification of picomoles of phenylalanine ammonia-lyase (PAL) reaction products. Separation has been achieved in 30 min on a reverse-phase silica column usingmethanol:water gradient elution, and PAD provided sensitive and specific determination of phenolics. Themethod has been applied to biological samples to study kinetin-induced changes in phenol pathways withinprotoplasts of Rubus fruticosus(raspberry). © 1997 by John Wiley & Sons, Ltd.Keywords: High performance liquid chromatography; photodiode array detector; cinnamates; benzoates; elicitation;kinetin.
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