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Abstract
The methodology for the separation of complex mixtures of longipin-2-ene-7β, 9α-diol-1-one diesters using high performance liquid chromatography followed by the identification of individual components by pulse Fourier transform 1H nuclear magnetic resonance spectroscopy is described. The identification methodology takes advantage of the spectral comparison between the isolated natural product and a wide series of previously prepared longipinene derivatives, and is applied to the study of the extracts of Stevia subpubescens var. intermedia which contain 11 previously isolated longipinene diesters and the new natural product longipin-2-ene-7β, 9α-diol-1-one 7-seneciate-9-angelate.
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