Abstract
A high-performance liquid chromatographic system employing a chemically bonded alkyl phase and a tetraalkylammonium phosphate buffer has been evaluated for separation of synthetic mixtures of hypothalamic oligopeptides. Separation of two peptides in the mixture having very similar adjusted retention times has been achieved. Sensitivity down to 5 ng is attained. Peptides range in size from three to 31 amino acids. Field desorption mass spectra illustrate novel structural elucidation methods for individual peptides and signal a quantification method.
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