High-performance liquid chromatographic separation of carbohydrates on a stationary phase prepared from polystyrene-based resin and novel amines

Abstract

New anion-exchange stationary phases O n ( n=1, 2 and 3) with a dimethylamino terminal functional group, where n is the number of oxyethylene units [–(CH 2CH 2O) n –], were prepared by the reaction of chloromethylated porous styrene–divinylbenzene copolymer beads and amines [(CH 3) 2N–(CH 2CH 2O) n CH 2CH 2–N(CH 3) 2]. HPLC separations of monosaccharides (sorbitol, fucose, glucosamine, mannose, glucose, galactose, fructose, allose and altrose) and disaccharides (trehalose, lactose, cellobiose and maltose) were performed successfully on these stationary phases. The ether group of the stationary phases O n was found to affect the separation of carbohydrates.