Abstract
The preparation of a chromatographic support showing similar selectivity and chemical inertness to hydroxyapatite (HA) and mechanical resistance to the pressures generally used in high-performance liquid chromatography is described. A mixed matrix was formed by covering macroporous silica microparticles with a thin layer of calcium phosphate (CaP—HA). The porous (500, 1000 and 4000 Å) silica microparticles (15 and 10 μm) had been previously inactivated with glycidoxypropyltrimethoxysilane in acidic medium, to convert the silanol groups into hydrophilic groups, producing a biocompatible support with excellent properties in terms of mechanical resistance to high pressure (5000 p.s.i.), selectivity, chemical inertness and efficiency. Columns packed with small particles (5 μm) were run at high pressure and high flow-rates for prolonged periods. Mixed silica WP-DIOL/CaP—HA matrices were used to separate a standard protein mixture antimelanoma monoclonal antibody and some small molecules such as carnitine derivatives and sugars.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
