Abstract
This paper describes the use of 2-(9-anthrylethyl) chloroformate (AEOC) as a sensitive and convenient pre-column, fluorophoric derivatizing reagent for the separation and detection of phenol (P), p-methylphenol (PMP), 3,4-dimethylphenol (DMP), and 4- tert.-butylphenol (BP) using reversed-phase high-performance liquid chromatography (HPLC) with fluorescence detection. Experiments were carried out to determine maximum fluorescence excitation and emission wavelengths, optimum derivatization pH, efficient gradient programming, calibration curves, minimum detection limits, and stabilities for AEOC-derivatized phenols. The method was applied to several industrial waste water samples from Central Pennsylvania. The minimum detection limits for injected phenol samples ranged from 7 to 10 n M, or 9, 10, 12 and 11 pg for P, PMP, DMP and BP, respectively. This corresponded to concetrations of phenols in the original waste water samples of 56 to 80 n M.
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