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Abstract
Procedures are described for the separation and quantitative estimation of choline and glycinebetaine aldehyde in plant saps and extracts. Glycinebetaine aldehyde in the plant material, and a suitable internal standard, were converted to their p-nitrobenzyl oximes during extraction. The extracts was purified on Sep-Pak C 18 cartridges and on ion-exchange columns, after which the choline fraction was benzoylated to yield UV-absorbing derivatives. High-performance liquid chromatography was performed on an aliphatic carboxylic acid or an aromatic sulphonic acid cation-exchange column eluted with the phosphate salt of choline in aqueous acetonitrile. The detection limit for both compounds was less than 1 ng. This procedure may also be applied to the analysis of choline in hydrolysates of phospholipids.
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