High-performance Liquid Chromatographic Method for the Quantification of Gallic Acid in Simhanada Guggulu

Abstract

Marker compounds quantification with new analytical tools and methods is necessary for establishing the authenticity and usage of Ayurvedic or herbal formulations. Simhanada guggulu or guggul is one of the supportive Ayurvedic medicines for treatment of rheumatoid arthritis and used in various disorders in Ayurveda such as limping, anemia, gout, disease of skin, cough, abdominal lump, pain digestive impairment. Simhanada guggulu is an Ayurvedic herbal formulation made by some selected herbs. The rejuvenating and tonic properties of ‘Simhanada guggulu’ are considered majorly due to their antioxidant principles, which in turn is due to the presence of phenolic compounds. A high-performance liquid chromatography (HPLC) method has been developed for quantitative determination of the gallic acid in ‘Simhanada guggulu’. The acidic mobile phase used in RP18 column which enabled efficient separation of gallic acid. A binary gradient with mobile phase containing solvent A (Acetonitrile) and solvent B (water: 0.3% O-Phosphoric Acid) was used for analysis. Elution was carried out at flow rate of 0.8 mL/min. Pure gallic acid Rt was found to be 5.29 min and peak with same Rt was also observed in prepared formulation. Gallic acid content of prepared formulation was found to be 2.28 %. The developed HPLC-UV method is simple, rapid and help as tool for the standardization of Simhanda guggulu.