Abstract
A rapid, simple and sensitive method for the quantitative determination of ticlopidine in the human serum has been developed. Ticlopidine and internal standard (5-(o-fluorobenzyl)-4, 5, 6, 7-tetrahydrothieno[3, 2-c]-pyridine maleate) were extracted from the alkalized serum. After purification on SEPPAK cartridge, the eluate of the cartridge was analyzed by high performance liquid chromatography by using Nucleosil 7C18 as a column packing and a mixture of 1/30 M phosphate buffer (pH 7.0) and acetonitrile (4 : 6) as a mobile phase. The recovery of added ticlopidine was more than 83% and the coefficient of correlation for the calibration curve in the concentration range of 0.06 to 3.53 μg/ml was 0.9998. Coefficients of variation in the same concentration range were 0.2-4.2%. The detection limit was 50 ng/ml in the human serum. It is conceivable that this method is accurate and useful for therapeutic drug monitoring.
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