Abstract
A simple a nd sensitive high per for ma nce liquid chromatography (HPLC) method was developed for qua ntification of clobeta sol (CLB) in r a t pla sma . Aripiprazole was used as an internal standard (IS). The present method uses protein precipitation with acetonitrile, followed by the liquid-liquid extraction with methyl tertiary butyl ether for extraction of the CLB from the matrix. Separation was carried out using Variance C 18 (250.0 × 4.6 mm, 5 μ m particle size) column and the effluent was monitored by an ultra violet (UV) detector at 240 nm. The mobile phase used was acetonitrile: phosphate buffer (pH 7.0; 25.0mM containing 0.2 %v/v triethylamine) (65: 35 % v/v) at a flow rate of 1.0 ml/min. The present method was va lida ted a s per the United States Food and Dr ug Administration (USFDA) guideline. This method was linear over the range of 25.0-1000.0 ng/ml with regression coefficient greater than 0.99. The mean recovery of CLB and IS were 70.8±1.9 and 83.32 ± 1.55 % respectively. The method was found to be precise, accurate and specific. The developed HPLC method was applied for the estimation of CLB in plasma after topical application of various zinc salts in rats to study the penetration of CLB through rat skin.
Highlights
IntroductionChronic, relapsing, genetically determined, inflammatory and proliferative disease of the skin
Psoriasis is a common, chronic, relapsing, genetically determined, inflammatory and proliferative disease of the skin
high performance liquid chromatography (HPLC)-grade water was produced in the laboratory by a Milli-Q purification system (Millipore Corp., Vienna, Austria) and Milli-Q water was used for the preparation of the mobile phase
Summary
Chronic, relapsing, genetically determined, inflammatory and proliferative disease of the skin. It affects about 2-3% of world population. Topical corticosteroids are the most frequently used medication for the treatment of mild-to moderate psoriasis in dermatological practice. These molecules are generally effective, safe, easy to use and relatively inexpensive. Skin penetration and permeation of the drug after topical administration depends on the physicochemical properties of the drug molecule and on the function of the skin as a transport
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
