Abstract
Objective: The study is aimed to develop a simple, isocratic and sensitive reversed-phase high-performance liquid chromatography method for the analysis of granisetron during transdermal permeation studies through porcine ear epithelium.
 Methods: A reversed-phase (C18) column was used with ultraviolet detection at 210 nm. Selected mobile phase contained 25% (v/v) of acetonitrile and 75% (v/v) of 0.25 mM potassium dihydrogen orthophosphate solution pH adjusted to 3.0 using 1% orthophosphoric acid solution.
 Results: Calibration curve showed good linearity over 0.1–30 μg/mL concentration range of porcine ear permeates in 80% ethanol and 20% water (blank permeates). The applicability of the method was demonstrated by the analysis of diffusion study samples of granisetron through porcine ear epithelium and the steady-state permeability flux (J) from ethanolic solution was found to be 4.707 µg/square cm/h.
 Conclusion: The developed method can be used in the analysis of diffusion study samples of granisetron transdermal formulations through the porcine ear epithelium.
Highlights
Granisetron is used in the treatment or prophylaxis of emesis and nausea
Post-chemotherapy and post-operative care and transdermal antiemetic therapy have the potential to ease the suffering of patients who are experiencing nausea and vomiting, resulting from their primary therapy and experiencing significant discomfort associated with their medical condition per se [3]
It is widely believed that passive transdermal diffusion occurs predominantly through the lipid phase of the skin due to the continuous lipid regions in the stratum corneum [9,10]
Summary
Granisetron is used in the treatment or prophylaxis of emesis and nausea It is a 5-HT3 receptor antagonist indicated to treat vomiting caused by cancer chemotherapy and radiation therapy or nausea induced during post-operative care. It works by blocking serotonin, a natural substance in the body that causes nausea and vomiting [1]. Post-chemotherapy and post-operative care and transdermal antiemetic therapy have the potential to ease the suffering of patients who are experiencing nausea and vomiting, resulting from their primary therapy and experiencing significant discomfort associated with their medical condition per se [3]. The promising method to diminish its adverse effects is to deliver the drug through skin
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