Abstract
Dispersive liquid–liquid microextraction (DLLME) coupled with high performance liquid chromatography by ultraviolet detection (HPLC–UV) as a fast and inexpensive technique was applied to the determination of imipramine and trimipramine in urine samples. Response surface methodology (RSM) was used for multivariate optimization of the effects of seven different parameters influencing the extraction efficiency of the proposed method. Under optimized experimental conditions, the enrichment factors and extraction recoveries were between 161.7–186.7 and 97–112%, respectively. The linear range and limit of detection for both analytes found to be 5–100ngmL−1 and 0.6ngmL−1, respectively. The relative standard deviations for 5ngmL−1 of the drugs in urine samples were in the range of 5.1–6.1 (n=5). The developed method was successfully applied to real urine sample analyses.
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