Abstract
This paper describes, for the first time, a high-performance liquid chromatographic method for the determination of thiocyanate anion as its pentafluorobenzyl derivative. The method is based on pre-column derivatization of thiocyanate anion with α-bromo-2,3,4,5,6-pentafluorobenzyl (PFB · Br). The derivative and chromatographic conditions were investigated. It is found that of different derivative solvents, such as acetone, acetonitrile and dichloromethane, acetone is the best. The chromatographic conditions were optimized by using a Zorbax ODS column and methanol-water (80:20, v/v) as mobile phase at a flow-rate of 1.0 ml/min and the eluent was monitored by a UV detector operating at 254 nm. The detection limit is 2.7 ng, and the method is both sensitive and selective. It has been successfully applied to the analysis of waste water of an electroplate and the saliva of regular smokers. Recoveries thus obtained were 93.7–102% and 92.1–101%, respectively.
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