Abstract
A sensitive, selective and simple post-column detection method for the determination of propylthiouracil (PTU) based on its sensitizing induction on iodine–azide reaction and the combination technique of high-performance liquid chromatography has been presented. The analysis was conducted in the optimum conditions for iodine–azide detection system and HPLC separation. The linear range, the lower limit of detection and quantification for PTU in urine were established at the levels of 0.4–1.0 nmol/ml urine, 0.3 nmol/ml urine and 0.4 nmol/ml urine, respectively.
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