Abstract
Two fluorescence labelling agents, 9-fluoroenylmethoxycarbonyl (Fmoc) hydrazide and 9-fluoroenylmethyl chloroformate, were used for the determination of neutral and amino monosaccharides, respectively, in glycoproteins. The derivatives were separated on a Cosmosil 5 C 18 column by isocratic elution with acetonitrile—water (30:70) and determined by using fluorimetric and ultraviolet detectors. The detection limits of sugar Fmoc-hydrazones and Fmoc-amino sugars were 0.05–0.4 pmol with fluorimetric detection at 270 nm (excitation) and 320 nm (emission) and 1–3 pmol for ultraviolet detection at 263 nm. The hydrolysis conditions of glycoproteins were studied and sugar components of transferrin and fetuin were determined.
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