Abstract
A rapid and simple high performance liquid chromatographic method for the determination of lactose in milk was developed. Samples were diluted with 0.5% perchloric acid and centrifuged, and an aliquot of the supernate was mixed with acetonitrile. Lactose was separated on a 10 micron particle-size silica column with aqueous acetonitrile as the mobile phase. The recovery of lactose from whole, skim, and chocolate milk averaged 99.2, 101.1, and 100.4%, respectively. Coefficients of variation for routinely performed duplicate determinations are between 1.0 and 1.5%.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
