Abstract
High-performance liquid chromatographic methods were developed for the determination of glycyrrhizin (G) and glycyrrhetinic acid (GA) in blood, plasma, bile, urine, and visceral tissues of rats. The detection limit for G and GA was 0.125μg/ml or /g wet weight. The precision and sensitivity of the assay appear to be satisfactory for determination of the levels of these compounds in biological materials. These determination methods were developed to study plasma disposition, tissue distribution, and biliary and urinary excretions of G and GA after a bolus i.v. administration of G or GA to rats. Plasma disposition followed a two-compartment model for both G and GA with β-elimination half-lives of approximately 50 and 80 min, respectively. The cumulative amounts of G and GA excreted in bile during 24h after an i.v. dose were 88.5 and 0.36% of the dose, respectively, and those excreted in urine were, respectively, only 4.8 and 0.03% of the dose. These compounds were distributed in visceral tissues at lower concentrations than in blood.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
