Abstract
A method is described for the quantitative determination of clopidol in chicken tissues and eggs by high performance liquid chromatography. Samples were extracted with methanol and the extract was passed through an alumina column and then purified by anion exchange column chromatography with 1% acetic acid in methanol as an eluent. In the case of chicken tissues, the eluate was further purified by alumina column chromatography. The solvent was evaporated off and the residue was dissolved in methanol containing caffeine as an internal standard. Clopidol was separated on a column packed with LiChrosorb RP-18 (5μm) by using acetonitrile-water (10:90, v/v%) as a mobile phase, with detection at 265nm. The recoveries of clopidol added to chicken tissues and eggs at levels of 0.1 to 0.5ppm were 95.1% and 96.8%, respectively. This method was applied to commercial chicken tissues and eggs.
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