Abstract
A simple, rapid, sensitive selective chromatographic method has been developed for simultaneous determination of ciprofloxacin hydrochloride and ornidazole in human plasma by using internal standard. The method depends on reverse phase high performance liquid chromatography. The plasma sample was extracted using methanol: formic acid (5.5:0.5, v/v). A concentration range from 100-400 ng/ml for both drugs was used for calibration curve. The percent recoveries of ciprofloxacin and ornidazole were found to be 71.49-75.68 and 73.78-83.1 respectively. The mobile used consist of acetonitrile: methanol: water: triethylamine (40:20:40: 1% v/v/v) and flow rate 1 ml/min in isocratic mode. The separation was carried out by UV detector at wavelength 300 nm. The stability of ciprofloxacin hydrochloride and ornidazole in plasma were confirmed during three freeze-thaw cycles (-20°C), on bench during 12 h, and post preparative stability study. The proposed method was validated statistically and by performing recoveries study for determination of ciprofloxacin hydrochloride and ornidazole in human plasma.
Highlights
Ciprofloxacin (Figure 1) [1-cyclopropyl-6-fluoro-1,4-dihydro-4oxo-7-(piperazinyl)-quinolone-3-carboxylic acid] is broad spectrum fluoroquinolone antibacterial agent
The literature survey revealed that variety of analytical methods reported for estimation of ciprofloxacin in human plasma and other biological fluids [3,4,5,6,7,8], spectrophotometry [9,10], HPLC [11,12,13], HPTLC [14], and spectrophotometric methods [15,16,17] have been reported for estimation of ornidazole alone as well as in combinations
No method was reported for simultaneous determination of ciprofloxacin hydrochloride and ornidazole in human plasma by RP-HPLC using liquid-liquid extraction
Summary
Ciprofloxacin (Figure 1) [1-cyclopropyl-6-fluoro-1,4-dihydro-4oxo-7-(piperazinyl)-quinolone-3-carboxylic acid] is broad spectrum fluoroquinolone antibacterial agent It is effective in the treatment of a wide variety of infections including infections of bones and joints, those caused by gram-negative pathogens. Use of ornidazole in combination with fluoroquinolone in the treatment of pelvic inflammatory disease and intra-abdominal infection It is an antimicrobial agent used in treatment of susceptible protozoal infections and anaerobic bacterial infection. A widely used technique of quantitation involves the addition of an internal standard to compensate for various analytical errors In this approach a known compound of a fixed concentration is added to the known amount of sample to give separate peaks in chromatogram to compensate for the losses of the compound of interest during sample pretreatment steps. In this method tinidazole is used an internal standard as it does not interfere with the peak area of ciprofloxacin hydrochloride and ornidazole
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
