Abstract
An HPLC method using indirect conductometric detection is proposed for the simultaneous determination of eight active ingredients in cough-cold syrups. It involves the use of an Ultrasphere 5 μm Spherical 80 Å Pore CN analytical column (250 mm × 4.6 mm) as the stationary phase with a mixture of water, acetonitrile and ethanol (38:60:2) containing 1 m M perchloric acid as the mobile phase. The active ingredients included bromhexine hydrochloride, chlorpheniramine maleate, codeine phosphate, dextromethorphan hydrobromide, diphenhydramine hydrochloride, ephedrine hydrochloride, papaverine hydrochloride and phenylephrine hydrochloride. Derivatization of the drugs is not required. The effect of background conductance on detector response, and the factors affecting column separation of the ingredients were studied. The detector responses for all the drugs are similar. The calibration graphs exhibited a wide linear concentration range of 0–500 μg/ml for a sample size of 20 μl with correlation coefficients of better than 0.999 for all the drugs under study. The relative standard deviation for 10 replicate measurements of the content of each drug in cough-cold syrups was always less than 3%. The method has been applied to determine the active ingredients in a number of cough-cold syrups.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
