Abstract
Leachates from a waste degradation experiment, containing ∼700–3700 mg C/l of dissolved organic matter (DOM), were analyzed by high-performance liquid chromatography (HPLC) and liquid chromatography (LC), using various separation strategies. Scaling up of the separation scheme to a semi-preparative scale, suitable for hyphenated techniques, was also investigated. Separations with reversed-phase columns suggested that ∼70–93% of the DOM was hydrophilic, and ion-pair chromatography of this fraction showed the presence of several discrete compounds. Labile and non-labile complexes were formed by adding 60Co radiotracer. Size-exclusion chromatography indicated that the DOM was primarily in the <1000–1500 Da molecular mass range.
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