Abstract
A method is described for the determination of bradykinin, neuropeptide K (NPK), and substance P in patients with atypical carcinoid syndrome. The developed method uses a combination of conventional and solid-phase extraction as well as high-performance liquid chromatographic techniques. A narrow-bore C18 column with ultraviolet detection is used (200 nm). The technique recovers bradykinin at a level of 98%, NPK at 96%, and substance P at 98% (when pure standards are dissolved) at concentration levels relevant to the atypical carcinoid syndrome. In biological samples, the recovery rate of bradykinin, NPK, and substance P drops to 88, 86, and 88% respectively. The overall analysis time is 150 min from receipt of samples. This method proves to be a valuable tool in the identification of neuropeptides and thus the diagnosis of atypical carcinoid syndrome, especially in puzzling cases with nonspecific symptoms.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.