High Performance Liquid Chromatographic Analysis of Sulfonamides in Livestock Products Using Matrix Solid-Phase Dispersion (MSPD) Method with Silica Gel

Abstract

The MSPD method with silica gel (Silica Gel-MSPD) was developed for the extraction of sulfonamides in livestock products. Residual sulfonamides such as sulfameradine (SMR), sulfadimidine (SDD), sulfamonomethoxine (SMM), sulfadimethoxine (SDM) and sulfaquinoxarine (SQO) were extracted from chicken muscle tissue, yolk and pork muscle tissue. A sample (0.5g) was mixed with 0.7g of silica gel and 1.5ml of acetonitrile, and the mixture was dried, washed with hexane and eluted with methanol or tetrahydrofuran (THF). An aliquot of the methanol or THE solution was subjected to HPLC for the determination of each sulfonamide. The recoveries of sulfonamides added to the chicken, yolk and pork were 78.1 to 93.1%, 74.6 to 86.2% and 69.6 to 87.1%, respectively. The limits of detection for the proposed method were 0.01ppm for SMR and SDD, 0.02ppm for SMM and 0.04ppm for SDM and SQO.In order to confirm the utility of the proposed method, analyses of commercial products were performed, and the result was compared with that obtained by MSPD with ODS. Consequently, 0.94ppm of SMM and 0.99ppm of SDM were detected in two samples of pork muscle by the proposed method, whereas the values detected by using ODS were 0.81 and 0.56ppm, respectively.