Abstract
Normal-phase chromatograms of retinal- and retinol isomers using various mobile phases are presented, showing that in n-hexane- tert.-butyl methyl ether and also in n-heptane- tert.-butyl methyl ether the elution order for retinol is 13- cis, 11- cis-, 9- cis, and all- trans-retinol, whereas in n-hexane-1,4-dioxane 11- cis- elutes before 13- cis- retinol. Assignment of these main retinal and retinol peaks was performed using pure crystals of isomers, measurement of absorbance spectra and analysis of NMR spectra. The new mobile phase n-heptane- tert.- butyl methyl ether allows best baseline separation of the commonly occurring isomeric forms in reasonably short analysis time. In addition, eight di- cis- and tri- cis-retinol isomers were tentatively identified. The chromatograms show that former identifications in the literature are inconsistent or wrong.
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