Abstract
Even-numbered oligosaccharides derived from hyaluronate containing glucuronic acid or N-acetylglucosamine in a non-reducing position, as well as the corresponding odd-numbered oligosaccharides with N-acetylglucosamine or glucuronic acid at the non-reducing terminus, were separated by high-performance liquid chromatography and identified at 206 nm. Using an amino-modified silica gel column and 0.1 M KH 2PO 4 (pH 4.75) as the mobile phase, complete separation up to the octasaccharides was performed within 21 min. The effects of using various concentrations of acetonitrile in the eluent and of using various solvent pH values on the separation and retention data of the oligosaccharides were studied in detail.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
