Abstract
A high-pressure liquid chromatographic system for the separation of amino acids and peptide-hormone hydrolysates is described. A modified high-pressure liquid chromatograph (Hewlett-Packard, model 1010B) equipped with an automatic gradient-generating system, and the applications of luorescamine and ion-exchange resins of particle size 8 ± 2 μm permit the detection of amino acids in the picomole range with good resolution. Compared with conventional amino acid analyzers, the times for separation are reduced by a factor of 3–4.
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