Abstract
High performance ion exchange chromatography using aqueous solvents is presented for the separation of proteins. Diethylaminoethyl(DEAE) resin for the anion exchange column and sulfopropyl(SP) resin for the cation exchange column were utilized. Proteins, having a molecular weight of 10000 - 190000 daltons and an isoelectric point of 3.9-10.7, were separated within 60 min by sodium chloride linear gradient elution. The protein was eluted according to its isoelectric point. These columns are capable of separating proteins with the biological activity in high speed, high resolution, large capacity and considerably high recovery.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.